NGS library preparation - Items - D4Science Catalogue

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NGS library preparation

In general, the core steps in preparing RNA or DNA for Next Generation Sequencing (NGS) analysis are: (i) fragmenting and/or sizing the target sequences to a desired length, (ii) converting target to double-stranded DNA, (iii) attaching oligonucleotide adapters to the ends of target fragments, and (iv) quantitating the final library product for sequencing.

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Item URL

http://data.d4science.org/ctlg/ORIONKnowledgeHub/429-6bd8bfb0-ba66-49f7-9891-76e58c2c7a59

References

Field	Value
Authors	Steven R. Head, H. Kiyomi Komori, Sarah A. LaMere, Thomas Whisenant, Filip Van Nieuwerburgh, Daniel R. Salomon, and Phillip Ordoukhanian
DOI	10.2144/000114133
Journal	Biotechniques
PublicationTitle	Library construction for next-generation sequencing: Overviews and challenges
PublicationType	Journal Article
PublicationYear	2014
Website	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4351865/pdf/nihms580820.pdf
ZoteroURL	https://www.zotero.org/groups/2344323/orion/items/itemKey/NTKJYPBW

Glossary Term Classification

Field	Value
Category	Sampling and laboratory testing; Data processing and analysis
ModifiedDefinition	false
Sector	Shared Definition

Additional Info

Field	Value
Provided by:	EJP ORION project
system:type	GlossaryTerm

Management Info

Field	Value
Author	taras_guenther
Last Updated	29 April 2020, 02:08 (CEST)
Created	3 September 2019, 13:08 (CEST)